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  • An international group of taxonomists representing the United States, Japan, China, Thailand, Chile, and Vietnam describe the results of a 1999 workshop on the taxonomy of the groups Sargassum, Gelidiales, Gracilariales, Kappaphycus/Eucheuma, and Halymenia. This book, the eighth in a series, contains a special section on the marine algae of Vietnam. The California Sea Grant College Program, in cooperation with the University of Hawaii Sea Grant College Program, sponsored the workshop. It was organized and hosted by the Oceanographic Institute in Nha Trang, Vietnam. The rationale behind the first workshop in 1984 was that progress in seaweed aquaculture and marine natural products chemistry would require a better understanding of commercially interesting species. In these times of accelerated loss of habitat and biodiversity, the series of workshops have had the added benefit of providing lasting records of many species, including drawings and photos. They have also provided a forum at which algal specialists could work together on common regional and international problems.

    Author(s): Isabella A. Abbott, Karla McDermid
  • This work shows the technical and economical aspects of seaweed farming for the production of phycocolloids or marine gums. Two different cultivation systems were used in four different sites inhabited by Wayúu fisherman communities in the townships of Cabo de La Vela and Carrizal, Guajira Peninsula, Colombia. The productivity and the growth rate of three commercial important macroalgae species, as well as the production costs, investment and returns of 0,5 ha marine farms, taking into consideration the design and construction of cultivation units made with cheap and available materials. The implementation of these farming systems could lead for the technological transfer of the locals. The income obtained through seaweed farming could benefit a large part of the coastal community as an additional and complementary cash crop to their traditional activities, including artisan fishing and goat rising, where the majority thrives in conditions of extreme poverty with the highest unmet basic needs index of the country. 

    Author(s): Raúl E. Rincones León, Diego A. Moreno Tirado
  • This research investigated the technical feasibility of cultivating local seaweed species from Perth region in potassium-fortified inland saline waters (ISW). Different levels of potassium fortification into the ISW were essential for their culture and out of six seaweed species studied, Ulva lactuca, Lomentaria catenata and two Sargassum spp. could successfully be cultured in 33 to 100% potassium-fortified ISW. Seasonality, nutrient enrichments, pH and temperature were able to influence different seaweed species to different degrees.

    Author(s): Ha Thi Thu Bui
  • The world-wide increasing demand for seaweeds and seaweed products as items of food and raw material for the manufacture of industrial products as agar, carraheenan, and alginate has been the main factor which has encouraged the development of production technologies of economic species of seaweeds. This paper is a brief review of the current production technologies for four tropical seaweed genera, namely. Eucheuma, Kappaphycus, Gracilaria and Caulerpa. The related production problems and needs are also described. 

    Author(s): Gavino C. Trono Jr.
  • As the earth’s population continues to rise, concern over the availability of resources is increasing. Hunger, health, and availability of fuels are just some of the problems the world will need to solve. Currently, traditional agriculture plays a central role in food and fuel production, but growing population limits availability of the farmland required by these crops. With these problems in mind, marine agronomy, using oceans to produce usable crops, such as seaweed, becomes an appealing option. Seaweed offers not only a source of food, but also has some useful applications in pharmaceuticals and biofuels.

    Author(s): Kurt A. Rosentrater
  • The purpose of this study is to provide an initial assessment of the technical and economic feasibility of cultivating seaweed offshore to produce biofuels. This report reviews the seaweed industry and the higher value products that could improve the economic attractiveness of seaweed biofuel production process. We review previous attempts at offshore seaweed culture for biofuels, the technical and economic challenges faced by those projects, and the lessons learned. Progress in offshore seaweed farming technology is also examined.

    We propose a concept for offshore seaweed cultivation that positions large seaweed farms in natural nutrient upwelling areas. This concept greatly simplifies prior proposals based on artificial upwelling of deep ocean waters for nutrient supply. We conclude with a technology road map that recommends future activities to move offshore seaweed culture from the present concept and vision to a future commercial reality.

    For the context of this report, “offshore” or “open ocean” growing conditions refers to growing seaweed in waters that are generally too deep for even giant kelp to survive on their own and that are free from the direct influence of land. Nearshore refers to habitats of sufficiently shallow depth to enable such seaweeds to attach and grow or which provide a sheltered environment for aquaculture operations. This report documents the long history of using seaweeds to meet human needs. The economic value of seaweeds worldwide is currently about $6 billion USD, primarily as food products, and also as hydrocolloids for the food and pharmaceutical industry, soil conditioners, animal feeds, and cosmetics. The total seaweed harvest is reported at 15.7 million metric ton wet weight (about one million ton dry weight) per year, of which almost 90% is produced by nearshore aquaculture production. Thus, seaweed farming is already a significant industry, with a sophisticated technological basis, ranging from the biotechnology to aquaculture, processing, and marketing of the many products derived from these plants.

    As the need for renewable energy continues to grow, seaweed farming has the potential to help meet future energy needs. The oceans cover over 70% of the Earth’s surface. Use of just 1% of that along the ocean margins could supply about 3.5 billion dry ton of new biomass annually, if the production rates already achieved in coastal seaweed farms in countries like China could be projected for open ocean systems. This is three-times the maximum amount of terrestrial biomass that can be reasonably collected annually in the U.S. Such systems would not competewith the availability of fresh water, land, and nutrients needed to sustain terrestrial agriculture.

    Large-scale open ocean seaweed farming for biofuels production was attempted in the 1970s and 1980s, but was not technically successful. However, the lessons learned from that earlier attempt, together with advances in open ocean engineering and the current energy economics, provide the basis and incentive to develop a novel approach to open ocean farming. Indeed, exploratory R&D activities in Japan, Korea, Denmark, Germany, and the United Kingdom, among others, are already pioneering new efforts in this area. Large-scale open ocean farming
    could be used to produce the next generation biofuels, in particular butanol, for which historical precedence exists, and also to increase the supply of higher value animal feeds and bioproducts.

    The technical and economic viability of seaweed biomass production for conversion into biofuels requires an understanding of the factors that limit their growth in nature and under managed aquaculture operations, the evaluation of processes for converting the biomass into biofuels, and a determination of the risk factors in a seaweed-to-energy pathway. As noted above, we propose a concept for offshore seaweed cultivation, which we call the “Offshore Seaweed Farm”. This would be based on one-km2 (100 hectare) dynamically positioned floating seaweed production platforms. A Marine Biorefinery would take the seaweed biomass and process it into biofuels and other products.

    Author(s): G. Roesijadi, A.E. Copping, M.H. Huesemann, J. Forster, J.R. Benemann
  • Microalgae-based biorefinery coupled with effluent treatment (MBBET) is an approach that makes biomass production economically advantageous. It can produce value-added products from a circular bioeconomy perspective for a variety of industries. Although the global microalgae market is growing at a rapid rate of 7.39% per year and 70–90% of world's technological information is consolidated in patents, this source of technical data is still underexamined in the microalgae field. Technological prospecting is a powerful tool that provides insight into expanding knowledge and innovation in companies, providing inputs for competitive intelligence, improving decision-making, and forecasting technological changes. Thus, this review aims to fill this research gap by presenting a bibliometric analysis of patents conjugated with a natural language processing (NLP) textmining approach to transforming raw big data into useful information. The state-of-the-art prospective patents on MBBET are disclosed, demonstrating the potential of this integration, its historic-technological trends, the different effluents treated, and bioproducts of market interest. The research was conducted using the Espacenet and Orbit® databases. The data were recovered from patent documents from the last 20 years using a combination of the keyword “Microalgae” and code C02F3 (Biochemical effluent treatment), where 422 documents were recovered from Espacenet and 664 from Orbit®. In this paper, we show that patents are a noteworthy source of information for the advancement of microalgal biorefinery areas, especially when they are compared to scientific articles. Although biotechnological potential of algal biomass is still underexploited, technological gaps and active technologies are highlighted. MBBET is a promising key to mitigate global environmental challenges and is an expression of the new paradigm of water-food-energy. Future challenges are proposed to sketch perspectives on new avenues for technological improvements. 

    Author(s): Luiggi Cavalcanti Pessoa, Kricelle Mosquera Deamici, Luiz Antonio Magalhaes Pontes, Janice Izabel Druzian, Denilson de Jesus Assis
  • Saccharina latissima is an economically and ecologically relevant kelp species in Europe and North America. In kelps, the sexuality is expressed during the haploid life stage and the microscopic gametophytes exhibit significant sexual dimorphism. To understand the sex-dependent impact of temperature on the gametophyte stage, we analyzed for the first time, gene expression profiles of male and female gametophytes at three different temperatures (4, 12, and 20°C) characteristic for the species distribution range by using RNA-sequencing. We identified several differentially expressed genes (DEGs) between sexes; while female biased genes were enriched in general metabolism and energy production, male biased genes function within cell cycle and signaling. In our study, temperature modulated sex-biased gene expression, with only a small percentage of DEGs consistently male (7%) or female-biased (12%) at the three temperatures. Female gametophytes responded stronger to higher temperatures than males, suggesting that males are more heat tolerant. Differences between S. latissima and other brown algal gender-dependent gene expression might mirror the different evolutionary and ecological contexts. Genomic information on kelp gametophyte is still scarce and thus this study adds to our knowledge on sex differences in abiotic stress responses in macroalgae at the transcriptomic level.
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    Author(s): Cátia Monteiro, Sandra Heinrich, Inka Bartsch, Klaus Valentin, Erwan Corre, Jonas Collén, Lars Harms, Gernot Glöckner, Kai Bischof
  • Saccharina sculpera is highly valued for human consumption and value-added products. However, natural resources of this kelp have decreased sharply and it is in danger of extinction. Resources recovery through cultivation is being trialed to enable the sustainable use of this species. In this study, the temperature range for survival and optimal growth of juvenile S. sculpera was identified and applied to field cultivation. This study investigated the survival and growth of juvenile S. sculpera under six temperatures (i.e., 5, 10, 15, 16, 18, and 20°C) and two light intensities (i.e., 20 and 40 µmol photons m-2 s-1) in an indoor culture experiment. In these experiments, the blade length decreased at 16°C under the both light intensities. The thalli died at 20°C and 20 µmol photons m-2 s-1, and at 18‒20°C and 40 µmol photons m-2 s-1. During the field cultivation, early growth of S. sculpera was highest at the 5 m depth and growth decreased as the water depth increased. When the initial rearing depth was maintained without adjustment throughout the cultivation period (from December to October), all the cultivated S. sculpera plants died during August and September. However, S. sculpera plants lowered from 5 to 15 m and grew to 90.8 ± 13.1 cm in July. The seawater temperature at 15 m depth was similar to the upper level of thermal tolerance demonstrated by juvenile S. sculpera in the indoor culture experiments (16°C or lower). The plants were subsequently lowered to 25 m depth in August, which eventually led to their maturation in October. The present study confirmed that improved growth rates and a delay in biomass loss can be achieved by adjusting the depth at which the seaweeds are grown during the cultivation period. These results will contribute to the establishment of sustainable cultivation systems for S. sculpera.

    Author(s): Soo Hong Kim, Young Dae Kim, Mi Sook Hwang, Eun Kyoung Hwang, Hyun Il Yoo
  • The relationship between distributional boundaries and temperature responses of some Northeast American and West European endemic and amphiatlantic rhodophytes was experimentally determined under varying regimes of temperature, light, and daylength. Potentially critical temperatures, derived from open ocean surface summer and winter isotherms, were inferred from distributional data for each of these algae. On the basis of the distributional data the algae fall within the limits of three phytogeographic groups: (1) the Northeast American tropical-to-temperate group; (2) the warm-temperate Mediterranean Atlantic group; and (3) the amphiatlantic tropical-to-warm temperate group. Experimental evidence suggests that the species belonging to the northeast American tropical-to-temperate group(Grinnellia americana, Lomentaria baileyana, andAgardhiella subulata) have their northern boundaries determined by a minimum summer temperature high enough for sufficient growth and/or reproduction. The possible restriction of 2 species (G. americana andL. baileyana) to the tropical margins may be caused by summer lethal temperatures (between 30 and 35 °C) or because the gradual disintegration of the upright thalli at high temperatures (>30 °C) promotes an ephemeral existence of these algae towards their southern boundaries. Each of the species have a rapid growth and reproductive potential between 15–30 °C with a broad optimum between 20–30 °C. The lower limit of survival of each species was at least 0 °C (tested in short days only). Growth and reproduction data imply that the restrictive distribution of these algae to the Americas may be due to the fact that for adequate growth and/or reproduction water temperatures must exceed 20 °C. At temperatures ≦15 °C reproduction and growth are limited, and the amphiatlantic distribution through Iceland would not be permitted. On the basis of experimental evidence, the species belonging to the warm-temperate Mediterranean Atlantic group(Halurus equisetifolius), Callophyllis laciniata, andHypoglossum woodwardii), have their northern boundaries determined by winter lethal temperatures. Growth ofH. equisetifolius proceeded from 10–25 °C, that ofC. laciniata andH. woodwardii from 5–25 °C, in each case with a narrow range for optimal growth at ca. 15 °C. Tetrasporelings ofH. woodwardii showed limited survival at 0 °C for up to 4 d. For all members of the group tetrasporangia occurred from 10–20 °C. The southern boundary ofH. equisetifolius andC. laciniata is a summer lethal temperature whereas that ofH. woodwardii possibly is a winter growth and reproduction limit. Since each member of this group has a rather narrow growth and survival potential at temperatures <5 °C and >20 °C, their occurrence in northeast America is unlikely. The (irregular) distribution ofSolieria tenera (amphiatlantic tropical-to-warm temperate) cannot be entirely explained by the experimental data (possibly as a result of taxonomic uncertainties).

    Author(s): Yarish, Charles C. van den Hoek, A. M. Breeman

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