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  • Different propagation techniques for cultivationof vegetativeGigartina skottsbergiifronds were testedusing a system of suspended ropes, to which inoculantswere attached. Our results showed that triangular fragmentsobtained from the circularG. skottsbergiithalli producedharvestable frond of 800 cm2after 8 months. In contrast,inoculants of intact juvenile fronds of comparable sizeneeded at least 10 months to reach the same size. A controlexperiment with spores developing on an artificial substrateshowed that 20 months were needed to reach a surface of500 cm2, confirming the superiority of our fragment culturesystem. A pilot study demonstrated that with a density ofsix fronds m−1of farming line, the proposed system can beeconomically interesting for local fishermen.

    Author(s): Renato Westermeier, David J. Patiño, Pedro Murúa, Juan C. Quintanilla, Juan Correa, Alejandro H. Buschmann, Ismael Barros
  • Next generation sequencing (NGS) technologies have revolutionized many areas of biological research due to the sharp reduction in costs that has led to the generation of massive amounts of sequence information. Analysis of large genome data sets is however still a challenging task because it often requires significant computer resources and knowledge of bioinformatics. Here, we provide a guide for an uninitiated who wish to analyze high-throughput NGS data. We focus specifically on the analysis of organelle genome and metagenome data and describe the current bioinformatic pipelines suited for this purpose.

    Author(s):
  • Various types of floating algae have been reported in open oceans and coastal waters, yet accurate and timely detection of these relatively small surface features using traditional satellite data and algorithms has been difficult or even impossible due to lack of spatial resolution, coverage, revisit frequency, or due to inherent algorithm limitations. Here, a simple ocean color index, namely the Floating Algae Index (FAI), is developed and used to detect floating algae in open ocean environments using the medium-resolution (250- and 500- m) data from operational MODIS (Moderate Resolution Imaging Spectroradiometer) instruments. FAI is defined as the difference between reflectance at 859 nm (vegetation “red edge”) and a linear baseline between the red band (645 nm) and short-wave infrared band (1240 or 1640 nm). Through data comparison and model simulations, FAI has shown advantages over the traditional NDVI (Normalized Difference Vegetation Index) or EVI (Enhanced Vegetation Index) because FAI is less sensitive to changes in environmental and observing conditions (aerosol type and thickness, solar/viewing geometry, and sun glint) and can “see” through thin clouds. The baseline subtraction method provides a simple yet effective means for atmospheric correction, through which floating algae can be easily recognized and delineated in various ocean waters, including the North Atlantic Ocean, Gulf of Mexico, Yellow Sea, and East China Sea. Because similar spectral bands are available on many existing and planned satellite sensors such as Landsat TM/ETM+ and VIIRS (Visible Infrared Imager/Radiometer Suite), the FAI concept is extendable to establish a long-term record of these ecologically important ocean plants

    Author(s): Chuanmin Hu
  • Four species of Porphyra, P. vietnamensis. P. suborbiculata, P. indica and £: kanyakumariensis have been reported definitely from the Indian coast. Porphyra kanyakumariensis is now reported at a number of stations along the Kerala coast. In addition, the protein, lipid and carbohydrate content of the species and hydrological parameters of the ambient waters are also given.

    Author(s): Chennubhotla, V S Krishnamurthy, Mathew, Susan, Imelda, Joseph
  • Biogeochemical processes occurring near the sediment–water interface can play an important role in the establishment and persistence of hypoxic-to-anoxic conditions in areas of moderate-to-shallow water depth. Results are given in this paper for diagenetic modeling of two sites from the area on the Louisiana shelf west of the Mississippi River Delta known as the “dead zone”. This is one of the largest and most studied regions where seasonal coastal hypoxia occurs. The diagenetic model was capable of generating good matches with depth profiles at both sites in the upper 8 cm. Moderate differences between predicted and observed concentrations below this depth are most likely due to the highly non-steady state conditions in this region. The model was also able to predict extremely low dissolved sulfide concentrations and bacterial sulfate reduction rates that were in good agreement with independent direct observations. A sensitivity analysis of the model to input parameters showed that the model was much more sensitive to changes in values under hypoxic conditions than norm-oxic or anoxic conditions in the overlying water.

    Simulations were carried out to first determine how the profiles of sediment porewater parameters and interfacial fluxes would change under differing quasi-steady state conditions where overlying dissolved oxygen concentrations and the rate of bioirrigation were varied. Next a non-steady state simulation was run to investigate how sediment biogeochemistry would change between these conditions during a hypothetical annual cycle. Results demonstrated a clear need to better understand the dynamic relationship among overlying water oxygen concentrations, the behavior of the benthic faunal community responsible for bioirrigation and sediment biogeochemistry.

    Author(s): John W. Morse, Peter M. Eldridge
  • Aqueous protein extracts from 30 Brazilian marine algae were examined for haemagglutinating activity using native and enzyme-treated rabbit, chicken, sheep and human erythrocytes. Most extracts agglutinated at least one of the blood cells used. Sheep and rabbit erythrocytes were more suitable for detection of the agglutinating activity. The minimum protein concentration necessary to produce positive agglutination was usually lower with enzyme-treated erythrocytes than native ones. The five algal protein extracts showing the greatest haemagglutination titre were tested for sugar-binding specificity. Only the activity present in the green alga Cauler pacupressoides was inhibited by simple sugars and not by the glycoproteins tested. The activity of the other four extracts was inhibited by at least one of the glycoproteins utilised.

    Author(s): Norma Maria Barros Benevides, Alexandre Sampaio, A. S. C. Lobato, W. R. L. Farias, F. H. F. Costa, D. I. A. Teixeira, A. L. P. Freitas
  • Halophila decipiens Ostenfeld, a new record for the Hawaiian Islands, was collected from three locations spanning the archipelago: Midway Atoll at 3–15m deep, O’ahu Island at 1–2m deep, and Hawai’i Island at 40m deep. These findings expand the biogeographical distribution of this pantropical seagrass into the north central Pacific Ocean. Specimens showed morphological, reproductive and DNA characteristics typical of the species.

    Author(s): D. Wilson Freshwater, Monica C. Gregoritza, Karla J. McDermid
  • Cultivation of macroalgae at the lowest trophic level, using only sunlight and nutrients from the sea while taking up CO2, will have a neutral carbon footprint and the biomass will contribute significantly to meet the demand for food, feed, materials, chemicals, fuels and pharmaceuticals in near future. Through a new bioeconomy based on cultivated macroalgae Norway will establish a future feedstock bypassing the competition with landbased agricultural resources and at the same time contribute to the replacement of fossil resources. This blue bioeconomy will strenghten Norway's role as the leading seafood nation as well as a leading supplier of marine, sustainable biomass. In order to boost a new bioeconomy based on cultivated macroalgae, three priority areas must be focused:

    • Biomass production technology

    • Biorefinery prosesses

    • Marked and product development

    Author(s): Jorunn Skjermo, Inga Marie Aasen, Johanne Arff, Ole Jacob Broch, Ana Carvajal, Hartvig Christie, Silje Forbord, Yngvar Olsen, Kjell Inge Reitan, Turid Rustad, Judit Sandquist, Roar Solbakken, Kristine B. Steinhovden, Bernd Wittgens, Robert Wolff , Aleksander Handå
  • A new floristic index, [I/H]RCP, for the estimation of seaweed flora depending on the seawater temperature, is proposed. The index is a ratio of [I]to[H]. [I] is the number of species including both of species with alternation of isomorphic generations and without alternation of generations, and [H] is the number of species including species with alternation of heteromorphic generations, for Chlorophyta, Phaeophyta and Rhodophyta. This new floristic index, [I/H]RCP, clearly demonstrates the positive relationships with seawater temperature which effects the change of seaweed flora.

    Author(s): Masahiko Miyata, Kunio Iwatsuki, Tomoko Tomizuka
  • The value proposition of algae technical metrics was the subject of a recent, industry-led, Focus Group interactive discussion. The interactive platform allowed for real time capturing and priority ranking of the topics that are deemed critical challenges for a future successful algae-based bioeconomy. This work ultimately sets the stage for providing guidance, methods, and standard reference materials tailored to respective algae industry segments for the cultivation, processing and marketing of high-quality and safe algae-based products. The group solicits input from the broader algae community on the value proposition and presents a case here to build a consensus around algae-specific metrics. The value proposition of technical standards to the industry is that guidance obtained through these collaborations builds producers’ and consumers’ trust that algae and algae-derived products are safe, reliable, and may allow for expanded market access, and increased competitiveness and international trade. 

    Author(s): Lieve M. L. Laurens

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