Digital library

  • Increasing algal cover on tropical reefs worldwide may be maintained through feedbacks whereby algae outcompete coral by altering microbial activity. We hypothesized that algae and coral release compositionally distinct exudates that differentially alter bacterioplankton growth and community structure. We collected exudates from the dominant hermatypic coral holobiont Porites spp. and three dominant macroalgae (one each Ochrophyta, Rhodophyta and Chlorophyta) from reefs of Mo’orea, French Polynesia. We characterized exudates by measuring dissolved organic carbon (DOC) and fractional dissolved combined neutral sugars (DCNSs) and subsequently tracked bacterioplankton responses to each exudate over 48h, assessing cellular growth, DOC/DCNS utilization and changes in taxonomic composition (via 16S rRNA amplicon pyrosequencing). Fleshy macroalgal exudates were enriched in the DCNS components fucose (Ochrophyta) and galactose (Rhodophyta); coral and calcareous algal exudates were enriched in total DCNS but in the same component proportions as ambient seawater. Rates of bacterioplankton growth and DOC utilization were significantly higher in algal exudate treatments than in coral exudate and control incubations with each community selectively removing different DCNS components. Coral exudates engendered the smallest shift in overall bacterioplankton community structure, maintained high diversity and enriched taxa from Alphaproteobacteria lineages containing cultured representatives with relatively few virulence factors (VFs) (Hyphomonadaceae and Erythrobacteraceae). In contrast, macroalgal exudates selected for less diverse communities heavily enriched in copiotrophic Gammaproteo- bacteria lineages containing cultured pathogens with increased VFs (Vibrionaceae and Pseudoal- teromonadaceae). Our results demonstrate that algal exudates are enriched in DCNS components, foster rapid growth of bacterioplankton and select for bacterial populations with more potential VFs than coral exudates. 

    Author(s): Craig E Nelson, Stuart J Goldberg, Linda Wegley Kelly, Andreas F Haas, Jennifer E Smith, Forest Rohwer, Craig A Carlson
  • The community organization of coral reef fishes in the seagrass sub-habitat of Kavaratti toll, Lakshadweep, India was studied during the period from January 1991 to June 1992. Twenty-seven families represented by 65 species were recorded by the visual censes method. The community diversity for families and species was 2.49 and 3.14 respectively. Juveniles and sub-adults of the most adult reef fish, which inhabit other sub-habitats, were recorded here. The high species diversity in the seagrass beds is due to their roles as nurseries, shelter and foraging grounds for many species.

    Labridae, Chaetodontidae, Acanthuridae and Mullidae were the most speciose families. Ocurrence of siganids was highly seasonal. The cover that seagrass canopy provides conceals many species and perhaps influenced counts. The occurrence of balistids could be related to the presence of interstitial and patches and abundant invertebrate food. Scorpaenids subsisted on abundant invertebrates and juveniles fishes. High counts and pronounced variations make seagrass beds unstable habitats. However, monsoon assemblages were relatively stable perhaps due to lack of excessive new recruits and a habitat shift by most species.

    Author(s): Vijay Anand, P E, Pillai, N G K
  • Excess nutrient loading to nearshore environments has been linked to declining water quality and ecosystem health. Macro-algal blooms, eutrophication, and reduction in coral cover have been observed in West Maui, Hawaii, and linked to nutrient inputs from coastal submarine groundwater seeps. Here, we present a forty-year record of nitrogen isotopes (δ15N) of intra-crystalline coral skeletal organic matter in three coral cores collected at this site and evaluate the record in terms of changes in nitrogen sources. Our results show a dramatic increase in coral δ15N values after 1995, corresponding with the implementation of biological nutrient removal at the nearby Lahaina Wastewater Reclamation Facility (LWRF). High δ15N values are known to be strongly indicative of denitrification and sewage effluent, corroborating a previously suggested link between local wastewater injection and degradation of the reef environment. This record demonstrates the power of coral skeletal δ15N as a tool for evaluating nutrient dynamics within coral reef environments.

    Author(s): Joseph Murray , Nancy G. Prouty, Sara Peek , Adina Paytan
  • The trade/lobbying group Cornucopia Institute has issued a ‘report’ that alleges a ‘smoking gun’ in carrageenan data published more than 10 years ago on an industry-supported website. The Cornucopia report was issued just as the National Organic Standards Board (NOSB) is conducting ‘sunset reviews’ of additives that may or may not be included in U.S. foods labeled as organic. The announcement of the report and the full-length report itself is fraught with anti-industry bias and fails even to accurately describe its research subject. The Cornucopia report is consistent with the group’s ideology and baseless attacks on carrageenan. Cornucopia believes in three central themes that are evident in all its carrageenan discussions: 1. If you cannot argue decades of peer-reviewed science, then argue the research funding. 2. If you cannot argue the truth, then allege conspiracy. 3. If all else fails, cherry-pick the data.

    Author(s):
  • Due to a current name change, European ʻUlva lactuca L.ʼ is now recognised under the name Ulva fenestrata Postels & Ruprecht (Hughey et al., 2019).  In this regard, the title of this publication should read “Chemical profiling of the Arctic sea lettuce Ulva fenestrata (Chlorophyta) mass- cultivated on land under controlled conditions for food applications”. Moreover, reference to the sea lettuce species in this study should be Ulva fenestrata throughout the manuscript. It is essential to push for the correct identification of the species because this has wider implications on the use of different species of sea lettuce for food. The authors would like to apologise for any inconvenience caused. 

    Author(s): Michael Y. Roleda, Sandra Lage, Daniel Fonn Aluwini, Celine Rebours, May Bente Brurberg , Udo Nitschke, Francesco G. Gentili
  • A cost analysis of aquatic biomass systems was conducted to provide the U.S. Department of ENergy with engineering cost information on which to base decisions in the area of planning and executing research and development programs dealing with aquatic biomass as an alternative energy resource. Calculations show that several hundred 100 square mile aquatic biomass farms, the size selected by DOE staff for this analysis, would be needed to provide meaningful supplies of energy. It should be noted that sustems which require wasts (sewage, power plant, CO2) as a carbon source, natural harbors or lakes, and natural upwelling sites may not have application to the present study simply because they are very small compared to the large needs projected by DOE.

    With this background, specific engineering analyses were conducted on two original design concepts for 100 square mile aquatic biomass system; outstanding experts in all aspects of this project were called upon to participate and provide information in projecting the costs for harvested aquatic biomass for these systems. 

    It was found that the projections of cost for harvested open-ocean biomass, utilizing optimistic assumptions of scientific and engineering design parameters, appear to be above any practical costs to be considered for energy. One of the major limitations is due to the need to provide upwelled sub-surface water containing needed nutrients, for which pumping energy is required. It is shown that for lower yields of biomass, the energy in ocean waves may marginally provide energy to pump suitable amounts of upwelled water for nutrient supplu; however, costs of harvested biomass growth at increasingly higher yields, so much nutrient containing water is required that environmental wabe energy is insufficient and fuel or electric power is required for pumping; thus, with high yields, obtaining a net there are very substantial environmental and legal aspects of aquatic biomass farming in general that appear especially ponderous for an open-ocoean system. 

    It is concluded from this analysis that large scale land-based aquatic biomass farms may merit development, but perhaps within a much narrower range than heretofore investigated. For example, land-based aquatic biomass systems based on biomass which require a carbon source other than carbon dioxide from the air appear to have higher costs than may be acceptable as an energy resource. Sewage slude appears to have limited availability as a carbon source for many energy farms and the utilization of CO2 from power plant stack gases requires duct work and distribution system which are prohibitively costly. 

    Author(s): Donald L. Wise, Elizabeth H. Wilson, Raplh L. Wentworth, A. Carl Sharon, Don C. Augenstein, Edward Ashare
  • A PDF on "CPI - Assets and Activities Relating to Seaweed".

    Author(s): Dr. Jerry Cooper
  • Crossing tests were made to determine the relationship between the identified Viva pertusa, which commonly grows in Japan as an attached form on exposed rocks, and the floating Viva forming "green tide" inside calm bays. The floating Viva thalli were collected from five major green tide sites in Japan (Yokohama, Mikawa, Miyajima, Kochi and Hakata). Reproductive maturation was induced in U. pertusa and the floating thalli from each site. Mating between induced gametes was observed. It is therefore believed that the floating thalli from Yokohama, Mikawa and Miyajima were mainly U. pertusa, while those from Kochi and Hakata were of a different species (Viva sp.l). Furthermore, the Viva species found in Mikawa is also a species (Viva sp.2) different from both U. pertusa and Viva sp. 1.

    Author(s): Goro Yoshida, Shigeo Kawaguchi, Masao Ohno, Masanori Hiraoka
  • Cultivated kelps and other macroalgae have great potential in future provision of food, feed, bioenergy, fertilizer, and raw material for a range of chemical products including pharmaceuticals, food and feed additives, and cosmetics. Only a few species are currently cultivated, almost exclusively in Asia. There is a range of species that could be utilized in different parts of the world, providing that protocols for reproduction, propagation, and cultivation are developed. Domestication of species involves selection of traits that are desirable in cultivation and in the utilization of the harvested biomass. Genetic improvement of cultivated species through recombination of alleles and selection (breeding) has ensured high productivity and product quality in both agri- and aquaculture and will likely do so for macroalgae cultivation and use as well. According to the published literature, genetic improvement of kelps in Asia has so far largely relied on utilization of heterosis expressed in certain combinations of parental material, sometimes species hybrids. Here, we explore and evaluate the various methods that could be used in kelp breeding and propose an initial simple and low-cost breeding strategy based on recurrent mixed hybridization and phenotypic selection within local populations. We also discuss the genetic diversity in wild populations, and how this diversity can be protected against genetic pollution, either by breeding and cultivating local populations, or by developing cultivars that are not able to establish in, or hybridize with, wild populations.

    Author(s): Franz Goecke, Gunnar Klemetsdal, Åshild Ergon
  • Water shortage is one of the leading global problems along with the depletion of energy resources and environmental deterioration. Recent industrialization, global mobility, and increasing population have adversely affected the freshwater resources. The wastewater sources are categorized as domestic, agricul- tural and industrial effluents and their disposal into water bodies poses a harmful impact on human and animal health due to the presence of higher amounts of nitrogen, phosphorus, sulfur, heavy metals and other organic/inorganic pollutants. Several conventional treatment methods have been employed, but none of those can be termed as a universal method due to their high cost, less efficiency, and non- environment friendly nature. Alternatively, wastewater treatment using microalgae (phycoremediation) offers several advantages over chemical-based treatment methods. Microalgae cultivation using wastew- ater offers the highest atmospheric carbon fixation rate (1.83 kg CO2/kg of biomass) and fastest biomass productivity (40–50% higher than terrestrial crops) among all terrestrial bio-remediators with concomi- tant pollutant removal (80–100%). Moreover, the algal biomass may contain high-value metabolites including omega-3-fatty acids, pigments, amino acids, and high sugar content. Hence, after extraction of high-value compounds, residual biomass can be either directly converted to energy through thermo- chemical transformation or can be used to produce biofuels through biological fermentation or transes- terification. This review highlights the recent advances in microalgal biotechnology to establish a biorefinery approach to treat wastewater. The articulation of wastewater treatment facilities with microalgal biorefinery, the use of microalgal consortia, the possible merits, and demerits of phycoreme- diation are also discussed. The impact of wastewater-derived nutrient stress and its exploitation to mod- ify the algal metabolite content in view of future concerns of cost-benefit ratios of algal biorefineries is also highlighted. 

    Author(s): Ayesha Shahid, Sana Malik, Hui Zhu, Jianren Xu, Muhammad Zohaib Nawaz, Shahid Nawaz, Asraful Alam, Muhammad Aamer Mehmood

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