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  • This study is the first to evaluate the effects of five seaweeds (Ulva sp., Laminaria ochroleuca, Saccharina latissima, Gigartina sp., and Gracilaria vermiculophylla) on gas and methane production and ruminal fermentation parameters when incubated in vitro with two substrates (meadow hay and corn silage) for 24 h. Seaweeds led to lower gas production, with Gigartina sp. presenting the lowest value. When incubated with meadow hay, Ulva sp., Gigartina sp. and G. vermiculophylla decreased methane production, but with corn silage, methane production was only decreased by G. vermiculophylla. With meadow hay, L. ochroleuca and S. latissima promoted similar methane production as the control, but with corn silage, L. ochroleuca increased it. With the exception of S. latissima, all seaweeds promoted similar levels of total volatile fatty acid production. The highest proportion of acetic acid was produced with Ulva sp., G. vermiculophylla, and S. latissima; the highest proportion of butyric acid with the control and L. ochroleuca; and the highest proportion of iso-valeric acid with Gigartina sp. These results reveal the potential of seaweeds to mitigate ruminal methane production and the importance of the basal diet. To efficiently use seaweeds as feed ingredients with nutritional and environmental benefits, more research is required to determine the mechanisms underlying seaweed and substrate interactions.

    Author(s): Ana R. J. Cabrita, Hugo M. Oliveira, Carla Mendonça, António J. M. Fonseca, Margarida R.G. Maia
  • The edible red seaweed (Kappaphycus alvarezii) is one of the algae species which was found to be rich in nutrients and nutraceutical. Hence, K. alvarezii may have the ability to suppress cancer through its antiproliferative properties. The aim of this study was to investigate the potential compounds of K. alvarezii, cytotoxicity properties of K. alvarezii extract on breast cancer cell line (MCF-7), investigated toxicity effect of high dosage K. alvarezii extract in rats and determined the effect of K. alvarezii on 7, 12- dimethylbenz[a]anthracene (DMBA) mammary carcinogenesis in rats. The method of LCMS/MS and MTT assay were used. For animal study, sub-chronic toxicity method was used, the rats were supplemented with 2000 mg/kg body weight daily of K. alvarezii crude extracts by oral gavage. For the anticancer effect of K. alvarezii crude extracts, this study consisted of three groups of the experimental, untreated and normal group of rats. The experimental and untreated groups of rats were induced with mammary tumour with DMBA. The experimental group of rats was given with K. alvarezii crude extracts orally. The results were being used to compare with the untreated group of rats and normal group of rats. All the rats were fed with standard diet and water ad libitum. Mortality, behavior changes and tumour sizes were observed specifically. The differences between the three groups of rats were evaluated by using the ANOVA test. By using LCMS/MS method, six unknown compounds were analysed. K. alvarezii crude extract reduced the cell viability of MCF-7 from 84.91% to 0.81% and the IC50 value is 4.1 0.69 mg/mL. For sub-chronic and heavy metal toxicity studies, no significant difference was found in haematological and biochemical values of the control group and experimental group. The growth rate of tumours in the untreated group of rats was found significantly higher than the experimental group of rats. Besides that, the white blood cells level in untreated group was found significantly higher than the experimental group and the normal group. In conclusion, K. alvarezii extract might able to slow down the growth rate of the tumour cells, therefore, identification of an active compound of inhibition growth rate of the tumour cells can be positively carried out in the future.

    Author(s): Vi-Sion Chang, Patrick N. Okechukwu, Swee-Sen Teo
  • The National Oceanic and Atmospheric Administration (NOAA) National Sea Grant College Program has been funding marine aquaculture projects since the inception of the program in 1968. Early funding emphasis was on disciplinary studies such as nutrition, pathology, genetics, systems engineering, and life history studies of promising candidate species for aquaculture.

    In the early 1990s, additional focus was placed on developing production systems that had the best potential for application in the environmentally conscious United States. These aquaculture technologies included recirculation system technologies, offshore aquaculture, and marine fisheries enhancement. Sea Grant funded several symposia on these subjects and proposed increases in funding through the NOAA budget process and this helped to further focus the research efforts for the NOAA based program.

    Author(s): James P. McVey
  • The present paper deals with the Indian seaweeds of economic value and the potential resources available in the inshore waters of the country. Results of the sample surveys carried out to estimate the standing crops of all marine plants growing in the Palk Bay area are given. Possibilities of cultivating the commercially valuable species in sheltered and calm areas of the coastline are indicated based on the culture experiments conducted with Gracilaria lichenoides and G. corticata.

    Author(s): Rao, M Umamaheswara
  • With seaweed resources certainly not inferior to those of Japan or any other country, and probably much superior, the United States may be. said practically to ignore these valuable products except at a few points on its extensive coast. Statistics recently gathered give the paltry sum of $35,000 as the value of the marine algre prepared in the United States in one year. The business is practically restricted to Massachusetts, and is addressed to a single species, the "Irish moss" {('hondrux crin- pus). Considerable quantities of seaweeds are used as fertilizer on farms adjacent to the coast, but this is not a commercial enterprise. In Monterey and Santa Barbara counties, Cal., the Chinese fishermen dry certain algse for food, medicine, and fertilizer; in 1899 the quantity prepared was 35,K24 pounds, valued at $896. There is undoubtedly a good opportunity to develop the seaweed industry of every section of the United States coasts, and to establish a profitable trade in the various species and preparations of marine alga? along the new lines indicated in the foregoing paper on the Japanese seaweed industry, as well as by increasing the output of the species already sparingly utilized. To this end the following information and suggestions are offered in regard to some of the useful alga? of the United States.

    Author(s): Hugh M. Smith
  • Our planet is supposed to host 11.213 billion people by the end of the year 2100. Such demographic explosion poses serious problems for human life quality and security. Generally, the term “seaweed” is conventionally used to designate multicellular marine algae. In the last three decades, and due to the high diversity of their metabolites, seaweeds are used in medicine to treat gall stones, stomach ailments, eczema, cancer, renal disorders, scabies, psoriasis, asthma, arteriosclerosis, heart disease, lung diseases, ulcers, etc. Compounds like carotenoid, polysaccharides, fatty acids, glycoproteins, haloforms, halogenated alkanes, alkenes, alcohols, aldehydes, hydroquinones, ketones, phlorotannins, pigments, lectins, alkaloids, terpenoids, sterols and some heterocyclic and phenolic compounds are among the most important seaweed substances that receive attention from pharmaceutical companies for use in drug development, or from scientists in the field of medical research. The potential pharmaceutical, medicinal and investigatory applications of these compounds in antibiotic, antiviral, anticancer, antioxidants, anti-inflammatory, anticoagulants, and antidiabetic production are discussed in this chapter.

    Author(s): Rai Abdelwahab
  • Physiological responses to the hypo-osmotic and temperature stresses of an invasive species, Gracilaria vermiculophylla, were compared to those of the native Atlantic species, G. tikvahiae and G. cervicornis. For the hypoosmotic and lower lethal temperature experiment, a Connecticut (CT) strain of the invasive G. vermiculophylla and a Rhode Island (RI) strain of the native G. tikvahiae were cultivated at combinations of five different salinities (S ¼ 5, 15, 20, 25 and 30) and at five different temperatures (58C, 108C, 158C, 208C and 258C) for 3 weeks. For the upper lethal temperature experiment, two strains of G. vermiculophylla from CT and Portugal, two strains of G. tikvahiae from CT and RI and one Florida strain of G. cervicornis were cultivated at temperatures ranged from 228C to 398C in 28C or 38Cincrements for 14 days. Gracilaria vermiculophylla showed a wide range of temperature (58C–348C) and salinity (5 – 30 S) tolerance; whereas, G. tikvahiae cannot withstand harsher environmental stresses, such as prolonged exposures to salinities of 20 S and temperatures of 108C or 348C. Gracilaria vermiculophylla also grew faster and had higher survival rates than G. tikvahiae or G. cervicornis. These results suggest that the high tolerance and growth capacity of G.vermiculophylla may be responsible for the successful invasion of this alga into Long Island Sound and elsewhere along the east coast of North America.

    Author(s): Jang Kyun Kim, Charles Yarish, Rui Pereira
  • Species of the green macroalgae genus Ulva often exhibit rapid growth, are generally cosmopolitan, and are rich in amino acids, vitamins, proteins, and minerals and have high potential for commercial uses. Ulva aquaculture was established and experimentally integrated into fish and shrimp farming in Brazil as Integrated Multi-Trophic Aquaculture projects. Decreases in fish farm production are often due to deaths caused by stress – with consequent increases in production costs. Essential amino acids, such as tryptophan and phenylalanine, have been used in fish farms as anxiolytic agents. In that context, a bibliographic survey was carried out to investigate advances during the last 17 years in the use of tryptophan and phenylalanine produced by Ulva species in fish farming. The biosynthesis patterns of tryptophan and phenylalanine were also examined in the research data. References to the presence of tryptophan and phenylalanine in Ulva spp. were encountered in 32 articles, with Ulva lactuca being the species most cited. References to the use of essential amino acids as anxiolytics in fish farming were encountered in 23 articles, with tryptophan being the most cited; none of the articles, however, mentioned the use of Ulva spp. as sources of anxiolytics. Temperature and pH were the factors that most influenced phenylalanine production. In conclusion, there is a potential role for the use of selected species of Ulva in fish farming as sources of tryptophan and phenylalanine for anxiolytic purposes.

    Author(s): Ana Carolina Calheiros, Renata Perpetuo Reis, Beatriz Castelar, Diana Negrão Cavalcanti, Valéria Laneuville Teixeira
  • Farmers are one of the most important components of any plant-based cultivation industry. The Philippines is one of the world’s major producers of red carrageenophyte algae and has tens of thousands of farmers involved in this industry. The production of algae such as Kappaphycus and Eucheuma increased significantly from the early 1970s, when the industry was established, before declining from the mid-2000s, due to a number of reasons, including disease and epiphyte outbreaks. The introduction of biosecurity measures has been one approach used to tackle this decline. Biosecurity-related knowledge, attitude and practices (KAP) of the seaweed farmers were assessed in the four highest seaweed producing regions in the Philippines: (1) Tawi-Tawi, (2) Palawan, (3) Zamboanga and (4) Bohol. Analyses showed that seaweed farmers from Tawi-Tawi had relatively higher KAP mean scores than the other three sites. Palawan and Bohol farmers, however, scored lower on their knowledge, higher on their attitude and highest on their practices compared with the other areas. Farmers from Zamboanga scored the lowest in both their attitude and practice mean scores, although their knowledge score was one of the highest. This is the first KAP assessment applied to the seaweed farming industry globally and the results, in which the farmers’ biosecurity-related knowledge and practices, which scored “Fair” (50–75%) across all the regions, and their attitudes, which scored predominantly “Good” (> 75%) suggest that there is potential to raise the score for biosecurity practices. This assessment highlights how biosecurity challenges are currently addressed by seaweed farmers in the Philippines and suggests how the KAP survey could be used as a tool by policymakers and scientists to address gaps in biosecurity management practices.

    Author(s): Jonalyn P. Mateo, Iona Campbell, Elizabeth J. Cottier-Cook, Maria Rovilla J. Luhan, Victor Marco Emmanuel N. Ferriols, Anicia Q. Hurtado
  • Aquatic organisms, such as microalgae (Chlorella, Arthrospira (Spirulina), Tetrasselmis, Dunalliela etc.) and duckweed (Lemna spp., Wolffia spp. etc.) are a potential source for the production of protein-rich biomass and for numerous other high-value compounds (fatty acids, pigments, vitamins etc.). Their cultivation using agro-industrial wastes and wastewater (WaW) is of particular interest in the context of a circular economy, not only for recycling valuable nutrients but also for reducing the requirements for fresh water for the production of biomass. Recovery and recycling of nutrients is an unavoidable long-term approach for securing future food and feed production. Agro-industrial WaW are rich in nutrients and have been widely considered as a potential nutrient source for the cultivation of microalgae/duckweed. However, they commonly contain various hazardous contaminants, which could potentially taint the produced biomass, raising various concerns about the safety of their consumption. Herein, an overview of the most important contaminants, including heavy metals and metalloids, pathogens (bacteria, viruses, parasites etc.), and xenobiotics (hormones, antibiotics, parasiticides etc.) is given. It is concluded that pretreatment and processing of WaW is a requisite step for the removal of several contaminants. Among the various technologies, anaerobic digestion (AD) is widely used in practice and offers a technologically mature approach for WaW treatment. During AD, various organic and biological contaminants are significantly removed. Further removal of contaminants could be achieved by post-treatment and processing of digestates (solid/liquid separation, dilution etc.) to further decrease the concentration of contaminants. Moreover, during cultivation an additional removal may occur through various mechanisms, such as precipitation, degradation, and biotransformation. Since many jurisdictions regulate the presence of various contaminants in feed or food setting strict safety monitoring processes, it would be of particular interest to initiate a multi-disciplinary discussion whether agro-industrial WaW ought to be used to cultivate microalgae/duckweed for feed or food production and identify most feasible options for doing this safely. Based on the current body of knowledge it is estimated that AD and post-treatment of WaW can lower significantly the risks associated with heavy metals and pathogens, but it is yet unclear to what extent this is the case for certain persistent xenobiotics

    Author(s): Giorgos Markou, Liang Wang, Jianfeng Ye, Adrian Unc

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