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  • An effective semi-continuous process was developed to grow a locally isolated green microalga Chlorella sp. on acidogenically digested swine wastewater in bench scale for improved algal biomass production and waste nutrient removal using central composite design (CCD). The influences of two key parameters, namely wastewater dilution rate (DR) and hydraulic retention time (HRT), on algal biomass productivity and nutrient removal rates were investigated. The optimal parameters estimated from the significant sec- ond-order quadratic models (p < 0.05) were 8-fold DR and 2.26-d HRT. The cultivating experiment in a bench-scale multi-layer photobioreactor with the optimized conditions achieved stable algal productivity and nutrient removal rates, which fitted the predictive models well. Moreover, relatively high and stable protein and lipid contents (58.78% and 26.09% of the dry weight, respectively) were observed for the col- lected algae sample, indicating the suitability of the algal biomass as ideal feedstock for both biofuel and feed production. 

    Author(s): Bing Hu, Wenguang Zhou, Min Min, Zhenyi Du, Paul Chen, Xiaochen Ma, Yuhuan Liu, Hanwu Lei, Jian Shi, Roger Ruan
  • Background: Natural polyphenols have potential therapeutic effects on colon-based diseases and gut microbial dysbiosis. However, the delivery of pure polyphenols to the colon has to overcome chemical instability, de- gradation, and metabolism in the upper gastrointestinal tract after oral ingestion. Dietary fibers have been exploited as microbiota-triggered release systems to protect polyphenols in the upper gut and specifically deliver them to the colon. 

    Scope and approach: This review focuses on the recent development of colon-targeted polyphenol delivery systems using encapsulation technologies based on dietary fibers for both food and pharmaceutical applications. The detailed characteristics and advantages of commonly used dietary fibers and the main mechanisms of en- capsulation preparation are discussed. The challenges of targeting the colon and the colonic health benefits of polyphenols are elaborated. In addition, the scope for specific modulation of gut microbiota by the selective combination of polyphenol and dietary fiber is highlighted. 

    Key findings and conclusions: The microbial-triggered release mechanisms of dietary fiber-based delivery systems maintain the structural integrity and protect the polyphenols during passage through the harsh environment of the upper gastrointestinal tract to maximize their concentration in the colonic region. In addition, dietary fibers offer several advantages over other materials for polyphenol encapsulation and delivery, including strong dietary fiber-polyphenol binding interactions, high colonic mucoadhesion, and synergistic prebiotic effects from dietary fiber and polyphenol that result in health benefits for the colon and the body. 

    Author(s): Hsi-Yang Tang, Zhongxiang Fang, Ken Ng
  • Brown seaweeds are popular foods in Japan, where the incidence of breast cancer is about 1/6 the rate of that reported for American women. Seaweed is an excellent source of fiber, contains iodine, carotenoids, and both mammalian lignans and isoflavones. Seaweed may help to prevent breast cancer by several different mechanisms involving these and other constituents. In a preliminary study of toxicity and efficacy using 5 g/day of seaweed, we found seaweed was well tolerated and was associated with some biological changes in the variables measured. In this study, we will examine the effects of escalating doses I of seaweed supplementation in a group of healthy postmenopausal women with and without breast cancer. In our cross-over design, women will be randomized to either seaweed or placebo first. We will then give doses of seaweed or placebo (3 g/day for 3 weeks, then 6 g/day for 3 weeks), followed by 1 week of 6 g/day seaweed/placebo plus soy (2 mg isoflavone per kg bodyweight). Our primary outcome variables are changes in circulating estrogen levels, thyroid hormones, and urinary excretion of phytoestrogens. Adherence to our program will be measured by monitoring urinary excretion of iodine.

    Author(s): Jane Teas
  • Background: Feed additives which can ease the negative effects of infection by the Aleutian mink disease virus (AMDV) are of interest to mink farmers. The effects of kelp meal (Ascophylum nodosum) supplementation on immune response, virus replication and blood parameters of mink inoculated with AMDV were assessed. AMDV-free black mink (n = 75) were intranasally inoculated with a local strain of AMDV and fed a commercial pellet supplemented with kelp meal at the rates of 1.5% or 0.75% of the feed or were kept as controls (no kelp) for 451 days. Blood was collected on days 0 (pre-inoculation), 31, 56, 99, 155, 366 and 451 post-inoculation (dpi). 

    Results: No significant difference was observed among the treatments for the proportion of animals positive for antibodies against the virus measured by the counter-immunoelectrophoresis (CIEP), viremia measured by PCR, antibody titer measured by quantitative ELISA, total serum protein measured by a refractometer or elevated levels of gamma globulin measured by iodine agglutination test at the sampling occasions. At the termination of the experiment on 451 dpi, there were no differences among treatments for antibody titer measured by CIEP, total serum protein, albumin, globulins, albumin:globulin ratio, alkaline phosphatase, gamma-glutamyl transferase, and proportions of PCR positive spleen, lymph node or bone marrow samples, but blood urea nitrogen and creatine levels were significantly lower in the 1.5% kelp supplemented group than in the controls. 

    Conclusion: Kelp supplementation improved kidney function of mink infected with AMDV with no effect on liver function, immune response to infection by AMDV or virus replication. 

    Author(s): A. Hossain Farid, Nancy J. Smith
  • Heat stress (HS) is the most potent environmental stressors for livestock in tropical and subtropical regions. HS induced splanchnic tissue hypoxia and intestinal oxidative damage, leading to endotoxemia and systemic inflammation. The present study evaluated and compared the modulatory effects of feeding Barki male sheep (Ovis aries) on a standard concentrated diet containing 2% or 4% of the brown seaweed (Sargassum latifolium) followed by roughage for 40 consecutive days on the toxicity-induced by exposure to severe environmental HS (temperature-humidity index 1⁄4 28.55  1.62). The present study showed that the diet containing Sargassum latifolium (especially 4%) modulated significantly (P < 0.05–0.001) almost all changes shown in the HS-exposed sheep including the increase in the thermo-respiratory responses (skin and rectal temperatures, and respiration rate) and the resulted dyslipidemia, anemia, and systemic inflammation (blood leukocytosis, the elevation in the erythrocyte sedimentation rate, and the increase in serum proinflammatory cytokines and heat shock protein-70 concentrations). In addition, Sargassum latifolium improved significantly (P < 0.05–0.001) the body-weight gain, kidney functions (especially at the high dose), and blood antioxidant defense system (total antioxidant capacity, and the activities of catalase and superoxide dismutase) in the HS-exposed sheep, as well as protected the animals from oxidative tissue damage and the risk of atherosclerosis. In conclusion, feeding sheep with the diet con- taining 4% of Sargassum latifolium was safe and suitable for animal nutrition, as well as efficiently alleviated the harmful effects of the environmental HS in Barki sheep through improving the animal antioxidant defense system, and regulating the thermo-respiratory and inflammatory responses. 

    Author(s): Ashgan M. Ellamie, Wafaa A. Fouda, Wael M. Ibrahim, Gamal Ramadan
  • Since it was introduced to Zanzibar (Tanzania), seaweed farming has significantly contributed to local, socio-economic development. However, several investigations have shown impacts on the coastal environment near where the farms are located. As many seaweed farms are located on seagrass beds, there is a risk that seaweed farming could affect seagrass beds, and thereby disturb important ecosystem functions and the flow of ecological goods and services. This study compares characteristics of macrophytes (focusing on seagrasses), benthic macrofauna and sediment in seagrass beds, with and without seaweed farms, and a sand bank without vegetation in Chwaka Bay, Zanzibar. The results showed that seagrass beds underneath seaweed farms generally had less seagrass and macroalgae, finer sediment, lower sediment organic matter content and a reduced abundance and biomass of macrofauna, than seagrass beds without seaweed farms. Further, the macrofaunal community structure in seaweed farms showed more similarities to that on the sand bank than in the unfarmed seagrass beds. Most of the dissimilarity was attributable to Lucinidae (suspension-feeding bivalves), which were almost absent in the seaweed farms, resulting in the large difference in biomass between the seaweed farms and the unfarmed seagrass beds. When interpreted together with information from farmers, the observed pattern is believed to be caused by the seaweed farming activities. This indicates that more research is needed to establish the effects of seaweed farming on seagrass beds, and that more attention should be given to the location of farms and the choice of farming methods.

    Author(s): Johan S Eklöf, Maricela de la Torre-Castro, L. Adelsköld, Narriman Saleh Jiddawi, Nils Kautsky
  • Macroalgal distribution and abundance in different regions of a coral reef was studied for a period of one year in 1998 at Minicoy Island, Lakshadweep. The biomass distribution of algac on the seaward side of the reef varied from 620.2 g wet wt/m2 in September to a maximum of 2800.6 g wet wt/m2 in August. Reef flat had a minimum in July (251 g wet wt/m2) and a maximum in December (2074.9 g wet wt/m2). Significant seasonal differences were noticed in the three regions with maximum biomass during monsoon on the seaward side and in the postmonsoon at reef flat and lagoon side of the reef. Laurencia ceylanica ornate (32.0%) were major algae of he reef flat and lagoon side of the reef. Minor algae observed on the reef were Gelidiella acerasa. Bocrgesenia forbesil, Sargassum duplicatum and Cladophoropsis zollingeri. Hydrographical parameters such as water temperature, salinity, nutrients, rainfall and hours of exposure of algac were monitored.

    Author(s): Mohamed, Gulshad, Nasser, A K V, Koya, C N Haneefa
  • Resources survey of algae and seagrasses in 63 estuaries and backwaters existing from Madras to Athankarai in Tamil Nadu and Pondichery was made during 1988-89. Among these water bodies, only 44 supported vegetation. Totally 36 species of algae belonging to 23 genera under the groups Chlorophyta, Phaeoph~ta,R. hodophyta and Cyanophyta, and 5 species of seagrasses belonging to 3genera were recorded from these estuaries. The agar yielding seaweeds Gracilaria arcuata and G. verrucosa and carrageenan yielding seaweed Hypnea valentiae occurred in harvestable quantities in some estuaries.

    Author(s): Kalimuthu, S, Kaliaperumal, N, Ramalingam, J R
  • The deep water area between Valinokkam and Kilakkarai in Tamil Nady coast covering an area of 200 sq.km. was surveyed for the distribution of marine algae and seagrasses during January 1989. Totally 33 species of marine algae were recorded of which 8 species belonged to Chlorophyta, 8 to Phaeophyta and 17 to Rhodophyta. Only one species of seagrass Halophila ovalis occurred in the whole area surveyed. The vertical distribution of seaweeds and their abundance were investigated. Hydrological data collected from the area surveyed are presented.

    Author(s): Kaliaperumal, N, Kalimuthu, S, Muniyandi, K, Ramalingam, J R, Pillai, S Krishna, Chennubhotla, V S Krishnamurthy , Rajagopalan, M S, Rao, P V Subba, Rao, K Rama, Thomas, P C, Zaidi, S H, Subbaramaiah, K
  • The deep water areas at Alantalai--Manapad (Southern Zone) and Vembar-Nallatanni Tivu (Northern Zone) region in Tamilnadu were surveyed during 1988 for seaweed resources employing 'SCUBA' diving technique. A total of 28 species of algae in the Southern Zone and 27 in the, Northern Zone were encountered. Majority of the seaweeds belonged to Rhodophycaae.

    Author(s): Rao, K Rama, Rao, P V Subba, Mal, T K, Subbaramaiah, K, Kaliaperumal, N, Kalimuthu, S, Muniyandi, K, Ramalingam, J R, Pillai, S Krishna, Chennubhotla, V S Krishnamurthy

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