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  • Molecular biotechnology of marine algae is referred to as the biotechnology on the identification, modification, production and utilization of marine algal molecules. It involves not only the manipulation of macromolecules such as DNA, RNA and proteins, but also deals with low molecular weight compounds such as secondary metabolites.

    In the last decade, molecular systematic researches to investigate the relationship and to examine the evolutionary divergence among Chinese marine algae have been carried out by Chinese scientists. For example, RAPD has been widely used in several laboratories to elucidate genetic variations of the reds, such as Porphyra, Gracilaria, Grateloupia and the greens such as VIva and Enteromorpha. Some important data have been obtained. The study on molecular genetic markers for strain improvement is now in progress.

    In 1990s, genetic engineering of economic seaweeds such as Laminaria, Vndaria, Porphyra, Gracilaria and Grateloupia has been studied in China. For Laminariajaponica, the successfully cultivated kelp in China, a model transformation system has been set up based on the application of plant genetic techniques and knowledge of the algal life history. Progress has been made recently in incorporating a vaccine gene into kelp genome. Evidence has been provided showing the expression of gene products as detectable vaccines.

    In the present paper, the progress of molecular biotechnological studies of marine algae in China, especially researches on elucidating and manipulating nucleic acids of marine algae, are reviewed.

    Author(s): Cheng-Kui Tseng, Peng Jiang, Song Qin
  • Nutrient loads from the land to the sea must be reduced to combat coastal eutrophication. It has been suggested that further mitigation efforts are needed in the brackish Baltic Sea to decrease nutrients, especially in eutrophic coastal areas. Mussel farming is a potential measure to remove nutrients directly from the sea.

    Mussels consume phytoplankton containing nitrogen (N) and phosphorus (P); when the mussels are harvested these nutrients are removed from the aquatic system. However, sedimentation of organic material in faeces and pseudo-faeces below a mussel farm consumes oxygen and can lead to hypoxic or even anoxic sediments causing an increased sediment release of ammonium and phosphate. Moreover, N losses from denitrification can be reduced due to low oxygen and reduced numbers of bioturbating organisms. To reveal if mussel farming is a cost-effective mitigation measure in the Baltic Sea the potential for enhanced sediment nutrient release must be assessed.

     

    Author(s): J. Stadmark, D.J. Conley
  • To conserve the natural stock and also to get consistent crop year after year, the seaweed collectors have to follow a suitable time-table. Attempts must be made by the seaweed based industries to exploit these seaweeds during the maximum growth periods from their places of occurrence in order to meet the raw material requirements and also to conserve the economically important seaweeds growing in Tamil Nadu coast.

    Author(s): Kaliaperumal, N, Kalimuthu, S
  • Ocean Afforestation, more precisely Ocean Macroalgal Afforestation (OMA), has the potential to reduce atmospheric carbon dioxide concentrations through expanding natural populations of macroalgae, which absorb carbon dioxide, then are harvested to produce biomethane and biocarbon dioxide via anaerobic digestion. The plant nutrients remaining after digestion are recycled to expand the algal forest and increase fish populations.

    A mass balance has been calculated from known data and applied to produce a life cycle assessment and economic analysis. This analysis shows the potential of Ocean Afforestation to produce 12 billion tons per year of biomethane while storing 19 billion tons of CO2 per year directly from biogas production, plus up to 34 billion tons per year from carbon capture of the biomethane combustion exhaust.These rates are based on macro-algae forests covering 9% of the world’s ocean surface, which could produce sufficient biomethane to replace all of today’s needs in fossil fuel energy, while removing 53 billion tons of CO2 per year from the atmosphere, restoring pre-industrial levels. This amount of biomass could also increase sustainable fish production to potentially provide 200 kg/yr/person for 10 billion people. Additional benefits are reduction in ocean acidification and increased ocean primary productivity and biodiversity.

    Author(s): Antoine de Ramon N‘Yeurt, David P. Chynoweth, Mark E. Capron, Jim R. Stewart, Mohammed A. Hasan
  • The red seaweed, Gracilaria tikvahiae McLachlan, was cultivated in open water farms in urbanized estuaries of Long Island Sound (26-30 psu of salinity) and New York City (20-25 psu), USA in 2011. Plants were harvested monthly from summer (August, 24°C) to fall (November, 13°C) and analyzed for total nitrogen, protein, and amino acid content. On a dry matter (DM) basis, nitrogen and protein significantly increased over the harvest period until October and then pla-teaued. Nitrogen increased from 22 ± 1 g kg-1DM in August to 39 ± 3 g kg-1DM in October (p < 0.001). Protein increased from 107 ± 13 g kg-1DM in August to 196 ± 5 g kg-1DM in November (p < 0.001). With two exceptions, amino acid concen-trations expressed on a crude protein (CP) basis were similar over the harvest period. Essential amino acids accounted for 48 ± 1%of all amino acids present with lysine and methionine averaging 56 ± 2 g kg-1CP and 18 ± 1 g kg-1CP, respectively. Histidine was underrepresented among essential amino acids and averaged 13 ± 1 g kg-1CP. Taurine ranged from 2.1 to 3.2 g kg-1DM. With its moderate levels of lysine, methionine and taurine, ocean farmed G. tikvahiaehas the potential of overcoming many nutrient deficiencies currently associated with terrestrial plant ingredients in alternative feeds for fish and shrimp.

    Author(s): Yarish, Charles Ronald B. Johnson, Jang K. Kim , Lisa C. Armbruster
  • Aspects of the nutrient-uptake physiology of Porphyra dioica (Brodie et Irvine) from Porto, Portugal were investigated under laboratory conditions. The capacity for uptake and accumulation of nitrogen (N) by P. dioica was determined for two different N sources, ammonium and nitrate (). The influence of the light–dark cycle and of the simultaneous presence of and , as well as the effects of phosphorus (P) enrichment, on the growth, nutrient uptake, and accumulation were also evaluated. Porphyra dioica was able to take up, accumulate, and grow equally well using both sources of nitrogen when presented separately. The photosynthetic pigment levels increased significantly with the increase of the availability of N, for both sources. The chlorophyll a content was higher in thalli that used as source of N, while this difference was not seen for phycobiliprotein content. When both N sources were available (NO3 : NH4 = 6 : 1), P. dioica preferentially removed , with a clear diurnal difference. During the light period, the algae removed 70% of the available, while only 35% was removed during the dark period. Phosphorus enrichment did not influence the growth rate or the amount of P removed from the medium, suggesting a limited capacity to store P. These results indicate that P. dioica is a good candidate for application in an integrated multi-trophic aquaculture (IMTA) system.

    Author(s): Yarish, Charles Rui Pereira, George P. Kraemer, Isabel Sousa Pinto
  • Seaweed Energy Solutions AS (SES) has reached an agreement to acquire 100 percent of Denmark\'s Seaweed Seed Supply AS, a move that sharply reinforces SES\' position as Europe\'s leading player in large-scale seaweed cultivation for renewable energy and other uses, including feed.

    Author(s): Aquafeed.com Staff
  • The nutritional values of protein concentrates (PCs) isolated from two subtropical red seaweeds, Hypnea charoides Lamouroux and H. japonica Tanaka, were evaluated in growing rats. The protein quality of the two seaweed PCs was determined by comparing the net protein ratio (NPR), true protein digestibility (TD), nitrogen balance (NB), biological value (BV), net protein utilization (NPU) and utilizable protein (UP) of the two seaweed PCs diet groups with those of the casein control group.

    There were no significant differences of NPR and BV in all diet groups. Although the values of TD (ranged from 90.5 to 90.6%), NB (ranged from 108 to 113 mg rat−1 day−1), NPU (ranged from 80.1 to 81.3%) and UP (ranged from 80.1 to 81.3%) of these two PCs were significantly lower than those of the casein control, they were comparable to those of other common plant PCs. The growth performance of rats fed the two PCs diets was satisfactory and both PCs had no adverse effect on the weight of their major organs. Together with their good protein quality as mentioned above, the PCs from the two red seaweeds under study could be a potential alternative protein source for human nutrition.

    Author(s): K. H. Wong, Peter C. K. Cheung, Put O. Ang, Jr.
  • Studies were conducted to evaluate nutritional qualities of two edible green seaweeds, Caulerpa lentillifera and Ulva reticulata, with a view to their utilization in human nutrition. The proximate composition, mineral and vitamin contents, free fatty acid, and amino acid profiles were investigated.

    Protein and ash contents were the two most abundant components in these seaweeds. Caulerpa lentillifera and Ulva reticulata contained 12.49%, 21.06% protein and 24.21%, 17.58% ash based on dry weight, respectively. Both seaweeds contained high amounts of minerals and balanced amino acid profiles. Regarding the Dietary Reference Intake, both kinds of seaweeds were notably rich in iodine. Caulerpa lentillifera was also rich in phosphorus, calcium, magnesium and copper, while Ulva reticulata was rich in potassium, manganese and ferrous.

    Comparisons to corresponding nutrient values in other seaweeds and some commonly consumed local vegetables, both seaweeds showed their potential of being health food for human diets or as source of ingredients with high nutritional values.

    Author(s): Pattama Ratana-arporn, Anong Chirapart
  • Palmaria palmata (Dulse) is a red seaweed that may be a potential protein source in the human diet. Its protein content, amino acid composition, and protein digestibility were studied with algae collected every month over a 1-year period. Significant variations in protein content were observed according to the season: The highest protein content (21.9 6 3.5%) was found in the winter–spring period and the lowest (11.9 6 2.0%) in the summer–early autumn period. Most of the essential amino acids were present throughout the year. After 6-hour in vitro digestion in a cell dialysis using porcine pepsin and porcine pancreatin, the digestibility of proteins from Palmaria palmata crude powder, represented by dialyzed nitrogen, was estimated at 29.52 6 1.47%. Relative digestibility was 56%, using casein hydrolysis as 100% reference digestibility.

    In vitro digestibility of proteins extracted in water was analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis using either bovine trypsin, bovine chymotrypsin, pronase from Streptomyces griseus, or human intestinal juice. Dulse proteins were hydrolyzed to a limited extent, which confirmed a rather low digestibility. Hydrolysis rate was higher with trypsin and lower with chymotrypsin compared with the two other enzymatic systems, pronase and intestinal juice, respectively. The association of algal powder and protein extract to casein and bovine serum albumin, respectively, produced a significant decrease in the hydrolysis rate of the standard proteins. In conclusion, the digestibility of Palmaria palmata proteins seems to be limited by the algae non-proteic fraction.

    Author(s): Anne-Valerie Galland-Irmouli, Joel Fleurence, Radia Lamghari, Michel Lucon, Catherine Rouxel, Olivier Barbaroux, Jean-Pierre Bronowicki, Christian Villaume, Jean-Louis Gueant

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