Digital library

  • Development of research in the field of chemical inhibition of colonisation of seaweed surfaces has been constrained by the lack of appropriate methods for testing realistic concentrations of potentially deterrent compounds. Here we extend earlier work (de Nys et al. 1998) on the red alga Delisea pulchra to 6 other Australian seaweed species to investigate whether these methods could be used more generally in studies of natural defences against biofouling. We compared the effects of surface extracts of D. pulchra, Caulerpa filiformis, Dictyopteris acrostichoides, Dilophus marginatus, Laurencia rigida, Solieria robusta and Pterocladia capillacea on the settlement of 2 ecologically rele- vant fouling species, and further compared the effects of surface extracts to those of non-polar, whole-cell extracts of the 7 seaweeds. We also measured the natural biofouling cover of these sea- weeds in a field survey and examined whether levels of biofouling on the seaweeds in the field are predicted by the activity of either the surface extracts or the whole-cell extracts of these species. The results from settlement tests with surface extracts at natural concentrations showed that 2 species, D. pulchra and C. filiformis, had non-polar metabolites on their surfaces in sufficient quantities to significantly inhibit settlement. These species also had significantly lower biofouling cover in the field compared to the other seaweeds. The results of the settlement tests with whole-cell extracts, however, demonstrated that all the seaweeds contain non-polar metabolites that inhibit settlement at concentrations lower than total whole tissue content and that no individual whole-cell extract was generally more inhibitory than the others. Therefore, we conclude that results from settlement assays with whole-cell extracts are poor predictors of natural antifouling roles of seaweed metabolites, and that such bioassays are of little use if the objective is to explore the chemical mediation of interactions between seaweeds and fouling organisms. We also conclude, that with careful choice of solvent and extraction time, the surface extraction procedure described here may be broadly useful for investi- gating the deterrent effects of seaweed surface metabolites against fouling organisms.

    Author(s): G. M. Nylund, P. E. Gribben, R. de Nys, P. D. Steinberg, H. Pavia
  • Colloidal solutions of silver nanoparticles (AgNPs) were synthesized by gamma Co-60 irradiation using different stabilizers, namely polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA), alginate, and sericin. The particle size measured from TEM images was 4.3, 6.1, 7.6, and 10.2 nm for AgNPs/PVP, AgNPs/PVA, AgNPs/alginate, and AgNPs/sericin, respectively. The influence of different stabilizers on the antibacterial activity of AgNPs was investigated. Results showed that AgNPs/alginate exhibited the highest antibacterial activity against Escherichia coli (E. coli) among the as-synthesized AgNPs. Handwash solution has been prepared using Na lauryl sulfate as surfactant, hydroxyethyl cellulose as binder, and 15 mg/L of AgNPs/alginate as antimicrobial agent. The obtained results on the antibacterial test of handwash for the dilution to 3 mg AgNPs/L showed that the antibacterial efficiency against E. coli was of 74.6%, 89.8%, and 99.0% for the contacted time of 1, 3, and 5 min, respectively. Thus, due to the biocompatibility of alginate extracted from seaweed and highly antimicrobial activity of AgNPs synthesized by gamma Co-60 irradiation, AgNPs/alginate is promising to use as an antimicrobial agent in biomedicine, cosmetic, and in other fields.

    Author(s): Nguyen Quoc Hien, Le Quang Luan, Bui Duy Du, Nguyen Thi Kim Lan, Nguyen Ngoc Duy, Le Anh Quoc, Dang Van Phu
  • The alginic acid content of some Indian brown algae has been studied by Valson (1955). Pillai (1957). Kappanna at. a/5. (1962) Umamaheswara Rao (1969). Umamaheswara Rao and Kalimuthu (1 972) and Kaliaperumal and Kalimuthu (1976). In recent years information on growth variations and mannitol and alginic acid contents in certain alginophytes was made available by the studies of Umamaheswara Rao (1969). Umamaheswara Rao and Kalimuthu (1972) and Kaliaperumal and Kalimuthu (1976) . In the present account variation in growth. and mannitol contents in Pad in. gymnospo,a observed over a period of 2 years (January 1975 to December 1976) are given.

    Author(s): Chennubhotla, V S Krishnamurthy, Kalimuthu, S, Ramalingam, J R, Kaliaperumal, N
  • Soy protein concentrate (SPC) is a key ingredient in fish feed and most of it originates from Brazil. However, the Brazilian soy industry has reportedly resulted in significant environmental problems including deforestation. Consequently, new sources for protein are investigated and protein extracted from farmed seaweed is considered an alternative. Therefore, we investigate how seaweed protein product (SPP) can compete against SPC as a protein ingredient for fish feed. The study uses the positioning matrix, cost analyses involving the power law, and uncertainty analysis using Monte Carlo simulations, and key research challenges are identified. The initial finding is that, with the emerging seaweed industry, the cost of producing SPP is too high to be competitive for fish feed applications. To overcome this challenge, two solutions are investigated. First, substantial investments in cultivation and processing infrastructure are needed to accomplish scale, and a break-even scale of 65,000 tonnes is suggested. The second but more promising avenue, preferably in combination with the former, is the extraction of seaweed protein and high-value seaweed components. With mannitol and laminaran as co-products to the SPP, there is a 25–30% probability of a positive bottom line. Researches on extraction processes are therefore a necessity to maximize the extraction of value-added ingredients. Over time, it is expected that the competitive position of SPP will improve due to the upscaling of the volume of production as well as better biorefinery processes.

    Author(s): Jan Emblemsvåg, Nina Pereira Kvadsheim, Jon Halfdanarson, Matthias Koesling, Bjørn Tore Nystrand, Jan Sunde, Céline Rebours
  • The growing population, decreasing arable land and fresh water supply questions the sustainability of terrestrial agriculture for securing safe nutrients supply, particularly starch- an essential ingredient for all staple foods. Here, we report the isolation, characterization and offshore production assessment of native starch from green seaweed Ulva ohnoi cultivated in seawater. Starch content varied from 1.59% to 21.44% depending on growth conditions and seasons. Our results show that nutrient starvation significantly increased the starch concentration up to 21.4% on dry weight basis. The extracted fraction contained 75.45% starch, and the starch extraction yield from the U. ohnoi biomass was 50.37%. Ulva starch granules are spherical, ovoid and irregularly shaped, 5–7 μm in size. Their gelatinization temperature is 69° C and they are susceptible to α-amylase and amyloglucosidase digestion. U. ohnoi biomass cultivated offshore for 13 months showed an average starch yield of 3.43 ton/ha/ year (t·ha−1 y−1 ). This study encourages the potential use of offshore produced biomass for sustainable starch supply as an alternative to current agricultural products, the production of which requires arable land and fresh water.

    Author(s): Meghanath Prabhu, Alexander Chemodanov, Ruth Gottlieb, Meital Kazir, Omri Nahor, Michael Gozin, Alvaro Israel, Yoav D. Livney, Alexander Golberg
  • Effect of environmental factors such as exposure to air and desiccation, salinity, temperature, light and photoperiod on tetra spore shedding in the red alga Gracilaria crassa was studied under laboratory condition. In the tetrasporic thalli exposed to air in shade and sun for different durations, maximum quantity of spores were liberated from the thalli in submerged condition in control experiment. Spore output was observed at salinity ranging from 10 to 40% with peak output at 30%. Spore discharge was found at temperature ranging from 20 to 40' C with maximum quantity of spore liberation at 25OC. Spore shedding was recorded at light intensity ranging from 10 to 100 p Em2S-I with peak discharge at 20 p Em-2S-I. Maximum spore release occurred in long day condition at 12 : 12 LD cycle.

    Author(s): Sukumaran, Soniya, Kaliaperumal, N
  • Hardly a day goes by without there being a news item warning us of the impending shortage of oil and what it is going to cost us — assuming we can get it! The belated realization that non-renewable liquid fuels are going to increase in price, and possibly even be rationed, is one of the main reasons why biomass is being looked into so seriously by so many of the developed countries. For the developing countries, the energy problem is as acute — if not more so. The ‘woodfuel crisis’ is revealing the long-term detrimental agricultural, social and economic consequences of deforestation.

    Author(s): D.O. Hall
  • Carrageenan seaweed farming based primarily on the cultivation of Kappaphycus and Eucheuma species has grown significantly in the Philippines and Indonesia in the last two decades. Growth has also taken place on a smaller scale in the United Republic of Tanzania and a few other developing countries. Thanks to attributes such as relatively simple farming techniques, low requirements of capital and material inputs, and short production cycles, carrageenan seaweed farming has become a favourable livelihood source for smallholder farmers or fishers and generated substantial socio-economic benefits to marginalized coastal communities in developing countries. However, further development of carrageenan seaweed farming needs to overcome various barriers and constraints such as inclement weather conditions, disease outbreaks, uncertain and fluctuating market conditions, lack of value-added products and value-adding activities in most of seaweed farming countries, low incomes of seaweed farmers in some countries, and occupational health hazards. With six country case studies and one global synthesis, this document attempts to provide a balanced assessment and comparison of the social and economic performance of carrageenan seaweed farming in different countries. Various issues related to seaweed–carrageenan value chains are highlighted. The technical and economic performance of a number of carrageenan seaweed farming cases are systematically evaluated and compared. The positive and negative social impacts of carrageenan seaweed farming are discussed. Issues related to governance and institutions in the sector are reviewed. Challenges and constraints faced by different countries in the future development of their seaweed industries are identified. The document also highlights a series of information and knowledge gaps that need to be filled in order to form a clearer vision of carrageenan seaweed farming development in the future and facilitate evidence-based policy decision-making and sector management.

    Author(s): Diego Valderrama, Junning Cai , Nathanael Hishamunda , Neil Ridler
  • The objective of this study was to determine the optimal blade size and timing to transplant seed-stock of Ecklonia cava Kjellman onto the reef structure. We used the modified artificial stepped reef structure. A total of 14 units (3.0 m length × 3.5 m width × 1.1 m height) were deployed 7-8 m deep under the water to examine the optimal blade size and timing to transplant seed-stock of E. cava onto the structures. Sporophytes of E. cava <1 cm in length were all died within 1 month of transplantation. The blades of 5-10 cm in length which were transplanted in March 2007 survived and grew well on the artificial reefs. Growth rates of 5-10 cm size class were higher than those of longer blade sporophytes (20-30 cm size class, transplanted in April) while the survival rates showed no difference between the classes of blade size. Both classes of 5-10 and 20-30 cm in length grew until July, and a reduction in size had occurred in September. These results indicate the importance of the blade size of E. cava and timing for successful transplantation of the seaweed on artificial reef structures.

    Author(s): Yarish, Charles Jang K. Kim, Hyung-Joo Jin, Byung Hwa Min, Hyun Il Yoo, Jung-Pyo Hong, Mi Seon Park, Jung Min Shim, Young Dae Kim
  • Finding renewable alternative energy resources for fossil fuels substitution has become very vital due to the serious challenges faced by humankind at present such as environmental pollution, greenhouse gas emissions, climate change, crude oil price volatility, and fossil fuels exhaustion. Macroalgae (seaweeds) are fast-growing marine plants, providing several harvests per year without the need for arable land, fertilizer, and fresh water. Various types of ecosystems like coral reefs, mangrove forests, and rocky shores can efficiently host the seaweeds production systems. These characteristics have made them highly suitable feedstocks for third-generation bioethanol production. Iran has a huge potential in renewable energy resources owing to its unique geographical location and climatic features. The country borders with the Caspian Sea in the north and with the Persian Gulf and the Gulf of Oman in the south. Seaweeds farming can also play a key role in mitigating air pollution, increasing employment rate, sustaining fossil fuel resources, bioremediating contaminated water, and improving marine ecosystem in the Persian Gulf and the Gulf of Oman. In the present article, macroalgae diversity, cultivation, and their conversion and upgrading technologies into bioethanol in Iran are scrutinized and discussed. Finally, the potential of Bushehr (the Persian Gulf) and Chabahar (the Gulf of Oman) coastlines for macroalgae cultivation is investigated. These locations receive the annual solar radiation in the range of 1680‒1753 kWh/m2 and the photosynthetically active radiation (PAR) in the range of 2.6‒2.71 GJ/m2 /year with 3051‒3311.9 h sunshine per annum. Furthermore, the nutrient-rich and calm water with relatively stable pH, salinity, and temperature make these coasts suitable for macroalgae farming. A potential yield up to 147‒153 t/ha/year can be obtained if proper native/engineered species, well-situated sites, and compatible cultivation techniques are selected.

    Author(s): Hamed Kazemi Shariat Panahi, Mona Dehhaghi, Mortaza Aghbashlo, Keikhosro Karimi, Meisam Tabatabaei

Pages