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  • Multifaceted and diverse energy sources will replace our once massive accumulations of energy reserves. One of these energy sources will be biomass and its natural products; in fact, it will most certainly be one of the essential elements in the complex of the future energy structure. Solar and chemical energy conversion, through biology as a practical energy conversion mechanism, has been extensively documented and reviewed; therefore, this discussion will be restricted to microbial fermentations with specific evaluations of the potentials for microorganisms to synthesize oily hydrocarbons as fermentation products. In biosynthesis, the acyclic hydrocarbons are referred to as fermentation products on the basis of the strict definition of fermentation as being those chemical energy yielding reactions that require organic components as electron acceptors. A generalized fermentation scheme is given in the figure. The scheme is purposely restrictive to emphasize products that are potential fuels. Each of the fermentation products represents a valuable energy form. The most efficient of these fermentation products, in terms of cost of production, cannot be fairly evaluated at this time because of the differences in cell cultivation requirements, product recovery, and most importantly, since many of these products via microbial fermentations are not yet sufficiently developed for commercial consideration. With increasing awareness of microorganisms which grow well or adapt to marginal, extreme or waste environments (taking into account the benefit value of these environments and the rising expenses of waste treatment) the distinct probability exists that the production costs in developing fermentation systems for fuel will become increasingly feasible and attractive. Although the compounds listed in the figure are acceptable fuels and are accessible through microbial processes, the obvious selection of a biochemical fuel for development cannot be determined at this time because not all systems have been adequately investigated. The competitive readiness of the different fermentation systems and the economics of producing each product as they become developed will automatically map out our course of action in years to come

    Author(s): T. G. Tornabene
  • The global demand for clean products obtained from biobased resources has increased significantly with the rapid growth of the world’s population. In this context, microbially-produced compounds are highly attractive for their safety, reliability, being environment friendly and sustainability. Nevertheless, the cost of the carbon sources required for such approaches accounts for greater than 60% of the total expenses, which further limits the scaling up of industries. In recent years, algae have been used in numerous industrial areas because of their rapid growth rate, easy cultivation, ubiquity and survival in harsh conditions. Over the past decade, notable advances have been observed in the extraction of high-value compounds from algae biomass (ABs). However, few studies have investigated ABs as green substrates for microbial conversion into value-added products. This review presents the potential of ABs as the substrates for microbial growth to produce industrially-important products, which sheds light on the importance of the symbiotic relationship between ABs and microbial species. Moreover, the successful algal-bacterial gene transformation paves the way for accommodating green technology advancements. With the escalated need for natural pigments, biosurfactants, natural plastics and biofuels, ABs have been new resources for microbial biosynthesis of these value-added products, resolving the problem of high carbon consumption. In this review, the fermentative routes, process conditions, and accessibility of sugars are discussed, together with the related metabolic pathways and involved genes. To conclude, the full potential of ABs needs to be explored to support microbial green factories, producing novel bioactive compounds to meet global needs. 

    Author(s): Fady Abd El-Malek, Marian Rofeal, Hossain M. Zabed, Abdul-Sattar Nizami, Mohammad Rehan, Xianghui Qi
  • Physical and chemical properties of biodiesel are influenced by structural features of the fatty acids, such as chain length, degree of unsaturation and branching of the carbon chain. This study investigated if microalgal fatty acid profiles are suitable for biodiesel characterization and species selection through Preference Ranking Organisation Method for Enrichment Evaluation (PROMETHEE) and Graphical Analysis for Interactive Assistance (GAIA) analysis. Fatty acid methyl ester (FAME) profiles were used to calculate the likely key chemical and physical properties of the biodiesel [cetane number (CN), iodine value (IV), cold filter plugging point, density, kinematic viscosity, higher heating value] of nine microalgal species (this study) and twelve species from the literature, selected for their suitability for cultivation in subtropical climates. An equal-parameter weighted (PROMETHEE-GAIA) ranked Nannochloropsis oculata, Extubocellulus sp. and Biddulphia sp. highest; the only species meeting the EN14214 and ASTM D6751-02 biodiesel standards, except for the double bond limit in the EN14214. Chlorella vulgaris outranked N. oculata when the twelve microalgae were included. Culture growth phase (stationary) and, to a lesser extent, nutrient provision affected CN and IV values of N. oculata due to lower eicosapentaenoic acid (EPA) contents. Application of a polyunsaturated fatty acid (PUFA) weighting to saturation led to a lower ranking of species exceeding the double bond EN14214 thresholds. In summary, CN, IV, C18:3 and double bond limits were the strongest drivers in equal biodiesel parameter-weighted PROMETHEE analysis.

    Author(s): Muhammad Aminul Islam, Marie Magnusson, Richard J. Brown, Godwin A. Ayoko, Md. Nurun Nabi, Kirsten Heimann
  • The purpose of this chapter is to provide an overview of the main sys- tems of microalgae production with highlights of biofuel production. The large-scale production systems (raceway ponds, horizontal tubular photobioreac- tors, and heterotrophic bioreactors) and small-scale photobioreactors (vertical and flat-plate photobioreactors) will be presented and discussed with a special emphasis on the main factors affecting its efficiency, biomass productivities reported in the literature, scaling-up, costs of construction and operation, and commercial appli- cations. Besides this, the recent developments in microalgae cultivation systems will be reviewed in their main aspects. Finally, the criteria for selecting an appropriate bioreactor for microalgae cultivation will be presented, as well as the pros and cons of each system will be discussed in this chapter.

    Author(s): Mariana Manzoni Maroneze, Maria Isabel Queiroz
  • The 2015 Conference of the Parties (COP21) marked a turning point for global actions to mitigate atmospheric greenhouse gases, reduce the carbon dioxide emissions from fossil fuel combustion, and stabilize the global climate. On the other hand, the increase in energy demand asks for renewable sources and robust systems to supply energy and obtain product diversity like that obtained from a petroleum refinery. A biorefinery is the sustainable processing of biomass into a spectrum of profitable products and energy. Microalgal biomass is considered one of the most promising biorefinery feedstock providing alternatives for different areas, such as food, feed, cosmetics and health industries, fertilizers, plastics, and biofuels including biodiesel, methane, hydrogen, ethanol. Furthermore, microalgae can also be used for the treatment of wastewater and CO2 capture. However, microalgal biofuels are not currently cost competitive at large scale and to develop a sus- tainable and economically feasible process, most of the biomass components should be valorized. High-value coproducts from microalgae include pigments, proteins, lipids, carbohydrates, vitamins, and antioxidants, and they can improve the process economics in the biorefinery concept. Therefore, mild and energy-efficient down- stream processing techniques need to be chosen to maintain product properties and value. In this chapter, the existing products and microalgae biorefinery strategies will be presented, followed by new developments, sustainability assessments, and techno-economic evaluations. Finally, perspectives and challenges of microalgal biorefineries will be explored.

    Author(s): Pierre-Louis Gorry, León Sánchez, Marcia Morales
  • Among the red algae, Kappaphycus and Eucheuma are the two most commercially important carrageenophytes farmed extensively in Southeast Asian countries; they rank top in production in terms of volume of raw material produced. The farming of carrageenophytes has emerged as a successful enterprise and provides a promising, alternative livelihood option for low-income, coastal communities in a number of countries. In 2014, carrageenophyte production worldwide, surpassed other red seaweeds and was placed at the top of the production rankings with 10.99 million MT wet weight (77% of total production of farmed red seaweed) and Indonesia being a major producer. However, over time, the productivity of the crop (carrageenophytes) has declined in some regions due to sourcing of seedlings from single, selected genetic stocks considered to have higher yield potential which resulted in strain fatigue, or loss of vigour. The incidences of disease and epiphytic infestations are on rise in recent times which have severely affected biomass production, as well as the yield and product quality (carrageenan). In order to circumvent the crop productivity issues arising from clonal propagation, the raising of planting materials from spores, derived through the red algal sexual reproductive cycle, has been initiated to support the sustainability of selected, farmed carrageenophytes. Alternatively, in vitro tissue culture techniques have also been explored to not only rejuvenate the vigour of seedlings (i.e. the out-planting material) but also to seek seedlings resilient to stress, disease and epiphytes to act as an invigorated mother stock. These efforts have succeeded to a great extent in the development of appropriate techniques for explant culture, callus induction, callus sub-culture and regeneration to micro-propagules with improved traits. The present chapter briefly summarizes the developments and success achieved in micro-propagation of Kappaphycus and Eucheuma and also provides pointers to both gaps and priority areas for future research required for the advancement of sustainable farming of these carrageenophytes.

    Author(s): C.R.K. Reddy, , , , and , Nair S.Yokoya, Wilson Thau Lym Yong, Maria Rovilla J. Luhan, Anicia Q.Hurtado
  • Seaweed cultivation is a growth market worldwide. Seaweed has multiple uses and is a promising resource to contribute to the societal challenges of food security and climate change in the future. However, the mechanisation of seaweed cultivation is essential for further growth, especially in Europe or comparable regions with high labor costs. This development is comparable to the mechanisation of land based agriculture which started with the Industrial Revolution. The seaweed industry will make a similar transition from small scale artisanal cultivation to large scale fully mechansised farming, and we expect this to happen withing the timespan of a few decades. This is going to take place at sea, in the hostile marine environment, and it has to take place in a sustainable way. IHC adressses this formidable challenge from its strenghts and maritime engineering background. Seaweed cultivation mechanisation knowledge is being developed and and combined with our profound understanding of marine engineering. This is necessary in order to realise equipment which fullJls its harvesting functionalities and survive the unforgiving sea environment. IHC MTI, the R&D centre of Royal IHC, has developed a Jrst prototype harvesting machine and tested it to try out and understand harvesting principles and also to demonstrate the potential of mechanised harvesting. The initial prototype realises a cost reduction of 50% and harvesting time reduction of 90%, even at this early stage without impeding sustainability aspects. This presentation exhibits the results of the initial trials with the harvesting prototype. In addition we adress the next steps and technological challenges to achieve mechanised seaweed farming.

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  • Slow to find a commercial foothold on the west coast, commercial kelp production is growing rapidly on the Atlantic seaboard where growers in Maine have been cultivating it successfully for several years.

    The Hood Canal project funded by a Paul Allen Grant is the first of its kind on the West Coast to investigate the potential of kelp to combat ocean acidification.  The first kelp seedlings of a five-year project were unfurled into Puget Sound’s Hood Canal in Washington this spring. The spores, or sori, are raised in tanks at NOAA’s Manchester Research Facility. Scientists from NOAA and the Puget Sound Restoration Fund will monitor the kelp and surrounding waters over time to gauge it’s efficacy at taking up carbon dioxide from the water column. 

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  • Research undertaken in West Coast states and British Columbia suggests the best entry level markets for high quality seaweeds are in North America food markets. These markets could be targeted by future Alaska marine plant businesses selling high-value specialty products. Long term, the experiences gained in domestic markets could then be directed at niche Asian marine plant markets.

    Alaska has an excellent reputation as a source of high quality, sustainable seafood with outstanding brand recognition. Building on this international brand, economic opportunities in specialty food markets for Alaska sea vegetables and fertilizer concentrates holds the highest potential for success among entrepreneurs and coastal communities alike. However, before scaled up operations occur, state resource agencies still need to learn more about the number, quantity, and types of commercial marine plants in Alaska, establish total allowable harvest limits for sustainable wild seaweeds, and help to identify financing opportunities to assist an emerging wild aquatic plant and mariculture industry.

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  • As world energy demand continues to rise and fossil fuel resources are depleted, marine macroalgae (i.e., seaweed) is receiving increasing attention as an attractive renewable source for producing fuels and chemicals. Marine plant biomass has many advantages over terrestrial plant biomass as a feedstock. Recent breakthroughs in converting diverse carbohydrates from seaweed biomass into liquid biofuels (e.g., bioethanol) through metabolic engineering have demonstrated potential for seaweed biomass as a promising, although relatively unexplored, source for biofuels. This review focuses on up-to-date progress in fermentation of sugars from seaweed biomass using either natural or engineered microbial cells, and also provides a comprehensive overview of seaweed properties, cultivation and harvesting methods, and major steps in the bioconversion of seaweed biomass to biofuels.

    Author(s): Na Wei, Josh Quarterman, Yong-Su Jin

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