The experiment was conducted to evaluate the potential of three species of seaweed namely Ulva sp., Gracilaria sp. and Kappaphycus sp. as a biofilter in the culture of rotifer Brachionus plicatilis. Instantenous growth rate (µ) of rotifer was used as indicator for the efficiency of these seaweeds as biofilter. The result showed that the utilization of Ulva sp. (p<0.01), Gracilaria sp. (p<0.05) and Kappaphycus sp. (p<0.05), at 7 g in 10 L seawater, produced a significant effect as compared with the control. The results also showed when the highest µ values were obtained, addition of the seaweed into the culture will reduce the effectiveness of the biofilter. The results obtained in this study indicated that Ulva sp. can be used as a biofilter.