The yield and physicochemical properties of carrageenan from Eucheuma isiforme harvested at the Yucatán coast were investigated. Carrageenan was extracted under different alkali concentrations (0, 1, 3, 5 and 7% KOH) and treatment durations (3, 4 and 5 h). Native carrageenan, extracted without KOH, had the highest yield (;44.6%) independently of treatment duration. After alkali treatment, carrageenan yield ranged from 35.3 to 31.8%. No significant differences in carrageenan yield were observed between 1 and 3% KOH. Native carrageenan had low viscosity values (39–57.0 cPs), whereas carrageenan extracted with 1% KOH at 3 and 4 h increased in viscosity (160.0–161.3 cPs). Alkali-treated carrageenan formed very weak gels (-50 g cm-2) in 1.5% solutions. The chemical analysis and FTIR spectra revealed a preponderantly iota-carrageenan extract. Extractions performed with 1% KOH for 3 h produced carrageenan with suitable properties to be considered as a substitute for traditional iota-carrageenan sources.