Influence of low and high temperature on diurnal periodicity in tetrespore output from Gelidium pusillum, Petrocladia heteroplatos and Gelidiopsis variabilis was investigated. In general, peak shedding of spores was delayed than the normal periodicity in G. pusillum and G. variabilis at low (0 and 20°C) as well as high (40°C) temperature. Temperature plays a major role in altering the peak liberation of tetraspores in a day in these three red algae.