Recently,theuseofmathematicaltools,suchasthelifecycleassessment (LCA) methodology for ecologically sound processes, with the purpose of estab- lishing a process designer involving the limits of “cradle to grave” in an efficient and flexible way with less subjectivity, has become an ambitious challenge to be won. Therefore, to generate biofuels with low atmospheric emissions and minimal energy requirements has become crucial to commercial competitiveness. Thus, the objective of this chapter is to approach the current situation of the different sce- narios of microalgal biofuels production by an evaluation of them via a life cycle assessment. The chapter is based on three main topics: (1) fundamentals for structuring a life cycle assessment, (2) biofuels data set reported in the literature, and (3) application of LCA in microalgae biofuels.