The impact of gravity of renal failure for the current study on human health is well known and as there is no specific pharmacotherapy for renal failure, undertaken to evaluate the effect of seaweed extract of Chlorococcumhumicola, belongs to chlorophyta and is a unicellular green algae. The algae were collected from Mandabam, Rameshwaram, Tamil Nadu, India. Marine algae have been the source of active compounds where, they are used for various applications. The medicinaluse in traditional medicine has been reported since time immemorial. The present study is on the marine algae Chlorococcumhumicolawas investigated. The ethanol, ethyl acetate and hexane extracts of Chlorococcumhumicola were analyzed and subjected to phytochemical to know the secondary metabolites present in the ethanol extracts and showed the better activity so it was subjected for in vitro Nephroprotective activity.