Biosugar Production from Kappaphycus alvarezii by Hydrolysis Method Using Fungi Trichoderma harzianum

Kappaphycus alvarezii is one of the natural resourcescontaininghigh carbohydrate, and it haspotential as an alternative raw material for sugar industry.This studyaimsto analyze the optimumconditions of the fungi Trichoderma harzianum in degradatingpolysaccharides from K. alvareziiintosugar. The seaweed was collectedin Takalar Sea,South Sulawesi, Indonesia. Thefungiwaspreviouslyactivated at 30°C for three days in 2%ofseaweed substrate. The efficiency of theT.harzianuminproducingbiosugar was evaluated under various concentrationsof inoculum(5%, 10%, 15%and 20%)and hydrolysis time (0h, 24h, 48h, and 72h). The result implied thatK. alvareziicontain 55,58% ofcarbohydratewhichwasdominated by starch 35.83% and cellulose 12.21%.Furthermore, the highestcontent of sugar indicated by12,27g/L of reducing sugar was obtained at the combination of20% of thefungi and 24h incubation.In conclusion, the fungi T. harzianum has ability to convert carbohydrate ofK.alvareziito sugar through hydrolysis process.