This research was conducted to see to what extent the production factors used by the polyculture system of Gracilaria sp and milkfish farmers in Luwu Regency affect the production of Gracilaria sp. This research was conducted by interviewing Gracilaria sp cultivators in Luwu Regency by taking samples from three districts. The results show that the R2 value is 0.689 which means that 68.9% of the production / harvest can be explained by the production input, while the remaining 31.1% is influenced by other factors. In addition, it is also seen that the Fcount value obtained is 38.294 while Ftable (0.05) is 2.50 at the 95% confidence level, which means that the production input has a significant effect on the production of Gracilaria sp. Meanwhile, partially each production input has an effect on production, except for fertilizer production input.